Microbiology Lab Exercise Help
LAB EXERCISE 2
In this exercise you have a case in which you need to identify the causative agent of disease. You need to perform several laboratories tests. The task is to select from several options which are the appropriate tests and explain why you are going to perform them.
First read the case, then answers the multiple choice questions. And then answers the following questions , which are the questions that you will be submitting in your Assignment Folder
1. What is the first thing you are going to do with your sample? 2. How would you describe colony #1? 3. How would you describe colony #2? 4. What test do you do next? Why you have chosen such test ? 5. What test do you do next? Why you have chosen such test ? 6. Explain, identify and describe the causative microorganism of the disease.
Identify the unknown
Case : A 45 year old female with previous rhinorrhea, pharyngitis, and cough visits her doctor with a 102o fever which appeared abruptly after a sudden shaking chill episode. She has chest pain and a productive cough with rust colored sputum. Auscultation demonstrates inspiratory rales and “tubular” breath sounds in the right lung. X-ray shows diffuse lobar consolidation of the right lung. Sample is from sputum.
I. Chose from the following multiple choice the best answer (looking at the pictures) and explain or justify your answers in each question.
1. What is the first thing you are going to do with your sample?
A. Perform a Gram staining
B. Streak for isolation on MacConkey agar and blood agar plate
C. Catalase Test
D. Coagulase Test
E. Citrate Test
Let’s work up colony #1 first, then come back for colony 2. 2. How would you describe colony #1? A. Alpha-hemolytic and small
B. Alpha-hemolytic and large
C. Gamma-hemolytic and large
D. Gamma-hemolytic and large
E. Beta- hemolytic and small
B. Beta-hemolytic and large
3. What will you do next with colony #1? A. Perform a Gram staining
B. Streak for isolation on MacConkey agar and blood agar plate
C. Catalase Test
D. Coagulase Test
E. Citrate Test
4. Does this mean the bacteria must be staphylococci?
A. Yes
B. No.
5. What test do you do next?
A. Perform a Gram staining
B. Streak for isolation on MacConkey agar and blood agar plate
C. Catalase Test
D. Coagulase Test
E. Citrate Test
6. What does this result mean? A. Staphylococcus
B. Streptococcus
C. Bacillus
7. What test do you do next?
A. Perform a Gram staining
B. Streak for isolation on MacConkey agar and blood agar plate
C. Catalase Test
D. Coagulase Test
E. Citrate Test
The results of the test for this organism are shown on the BOTTOM. Some bacteria from a colony were mixed with rabbit plasma and incubated overnight. The tube at the top is provided for comparison as a control.
8. This result means A. Staphylococcus epidermidis
B. Staphylococcus aureus
II. Here again is an image of the blood agar plate from case 2. Now let’s work up colony type #2.
1. How would you describe colony #2?
A. Alpha-hemolytic and small
B. Alpha-hemolytic and large
C. Gamma-hemolytic and small
D. Gamma-hemolytic and large
E. Beta- hemolytic and small
B. Beta-hemolytic and large
2. What will you do next with colony #2?
A. Perform a Gram staining
B. Streak for isolation on MacConkey agar and blood agar plate
C. Catalase Test
D. Coagulase Test
E. Citrate Test
3. What does this test show? A. Gram-positive cocci.
B. Gram- positive cocci in a chain
C. Gram-positive cocci in pairs.
.
4. Does this mean the bacteria must be streptococci?
A. Yes B. No.
What test do you do next? A. Citrate test
B. Coagulase test
(The result of the test is negative) C. Catalase test.
5. What does this result mean? A. Staphylococcus
B. Streptococcus
6. What test do you do next? A. Oxidase test
B. Optochin (P disk)
C. Citrate test.
D. Motility test.
E. Methyl Red.
7. What does this result means?
A. Sthaphylococcus aureus
B. Staphyloccous epidermitis
C. Streptococcus pneumonia
Explain why you have chosen the microorganism.
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