Lab 11: Frog & Fetal Pig Dissections

Virtual Lab: Virtual Frog Dissection

http://mhhe.com/biosci/genbio/virtual_labs/BL_16/BL_16.html

1. If you come upon any terms that are unfamiliar to you, please refer to your textbook for further explanation or search the word here: http://encarta.msn.com/encnet/features/dictionary/dictionaryhome.aspx

2. In this exercise, you will be performing a virtual frog dissection. To begin, click on the “Introduction” link on the opening page. Read through and listen to the information presented to learn about the basics of dissection and animal phylogeny. When you are finished, click the “Menu” button at the bottom of the page to return to the opening page of the laboratory activity.

3. Once you are back to the opening page, click the “External Anatomy” button. Read through, watch and listen to the information presented in these segments. When you are finished, click the “Menu” button at the bottom of the page to return to the opening page of the laboratory activity.

4. The last portion of this activity involves an examination of the internal anatomy of a frog. To do this, click the “Internal Anatomy” button on the opening page of the laboratory. Read through, watch and listen to all of the information presented in these segments and actively participate where required. You may have to do a virtual cut on the frog by dragging the appropriate tool to the frog or label organs of the dissected frog by dragging the appropriate organ names to the site on the opened frog. Please STOP when you are through with each labeling step and be sure to fill in your final answers on Figures 1 and 2 below.

 

Figure 1: Digestive System OrgansFile:Diagram of pork cuts on a pig.jpg - Wikipedia

A   =

B   =

C   =

D   =

E   =

F   =

 

Figure 2: Organs   of the Circulatory, Respiratory, Excretory and Reproductive Systems

Frog Excretory System Diagram | Quizlet

A   =

B   =

C   =

D   =

E   =

F   =

G =

H =

Frog Dissection Post-laboratory Questions:

1.  The dorsal side of the leopard frog:

a.  Is a light, solid color

b. Is a colored and patterned

c. Is initially cut during a dissection

d. A and C

2.  Leopard frogs:

a.  Are invertebrates

b. Are warm-blooded

c. Have a gills at one time during their life cycle

d. All of the above

3. In regards to the external anatomy of a leopard frog:

a.  It is easy to tell the sex of the animal

b. The cloaca is at the anterior end of the animal

c. The feet of the hind limbs have 5 toes

d. All of the above

4.  In the opened mouth of the leopard frog, one can see:

a.  The nostrils

b. The glottis

c. The vomerine teeth

d. A and B

e. All of the above

5.  Which of the following is found in the digestive system of the leopard frog but not in that of a human?

a.  Gall bladder

b. Stomach

c. Pancreas

d. Liver

e. None of the above

6.  Arteries in the circulatory system:

a.  Carry blood to the heart

b. Carry blood away from the heart

c. Carry out diffusion of gases

7.  In the leopard frog heart:

a.  The right atrium carries oxygen rich blood

b. The left atrium carries oxygen poor blood

c. There are 3 chambers present

d. All of the above

8.  By comparison to the leopard frog heart, the human heart:

a.  Has 4 chambers present

b. Carries mixed blood in the ventricles

c. Is more efficient

d. A and C

e. All of the above

9.  Fat bodies play a role in:

a.  Respiration

b. Circulation

c. Hibernation

d. Reproduction

e. C and D

10.  The most anterior portion of the leopard frog brain is/are the:

a.  Olfactory lobes

b. Cerebrum

c. Optic lobes

Virtual Lab: Virtual Fetal Pig Dissection

https://www.whitman.edu/academics/departments-and-programs/biology/virtual-pig

Figure 1: Fetal Pig: Digestive System

1. __________________________
2. __________________________
3. _________________________
4. _________________________
5. ________________________
6. _________________________
7. __________________________
8. __________________________
9. __________________________
10. _________________________
11. _________________________
12. _________________________
13. ________________________

Identify the organ (or structure)

14. _____________________________ Opening (valve) between stomach and small intestine.
15. _____________________________ Stores bile, lies underneath the liver.
16. _____________________________ A branch of the large intestine, a dead end.
17. _____________________________ Separates the thoracic and abdominal cavity
18. _____________________________ Membrane that holds the coils of the small intestine.
19. _____________________________ The straight part of the small intestine, after stomach.
20. _____________________________ Empties bile into the duodenum from the gall bladder.
21. _____________________________ The last stretch of large intestine
22. _____________________________ Bumpy structure under the stomach; makes insulin
23. _____________________________ Lies between the two umbilical vessels.

Figure 2: Urinary and Reproductive Systems

Male  Female

Identify the Organs (or structure) on the above diagram:

A.

B.

C.

D.

E.

F.

G.

H.

I.

J.

K.

Identify the organ:

· Urine passes through the _________ to reach the urinary ___________ for storage.

· Urine exits the ________ to the outside through the ____________

· The ______________ is where sperm production takes place.

· The ______________ contain all the developing eggs the female pig will ever have.

· Called a ___________________ in humans, the __________ receives the egg from the ovary at ovulation.

· Fertilized eggs travel from the oviducts into the ______________ for implantation.

 
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Genetics 303

Genetics 303

Fourth exam—take home 2019

Answer on SEPARATE paper, show all work, and be neat in the reporting of answers. It is easier to grade if you try to

keep the questions in sequence. STAPLE YOUR RESULTS!

1. In a human population, the genotype frequencies at one locus are 0.75 AA, 0.20 Aa, and 0.05 aa. What is the frequency

of the A allele [f(A)] and a allele [f(a)] for the population? Are they in Hardy-Weinberg equilibrium? (5 pts)

2. Calculate the number of heterozygotes in a population with p = 0.6 and q = 0.4 (at time = 0). After 4 generations of

inbreeding between siblings (F = 0.25) in a population of 650. (6 pts)

3. Human albinism is an autosomal recessive trait. Suppose that you find a village in the Andes where 8/1000 of the

population is albino. If the population size was 1600 and the population is in Hardy-Weinberg equilibrium with

respect to this trait, how many individuals are expected to be carriers (heterozygotes)? (5pts)

4. A boatload of 400 Swedish tourists, all of whom bear the MM blood group genotype, are marooned on Haldane Island,

where they are met by an population of Islanders totaling 1000, all bearing blood group NN genotype. In time, the

castaways become integrated into Island society. Assuming random mating, no mutation, no selection (based on

blood group), and no genetic drift, what would you expect the blood group distribution to be among 320 progeny of

the new Haldane Island population? (5 pts)

5. You identify a population of mice (Peromyscus maniculatus) on an island. Their coat color is controlled by a single

gene: BB mice are black, Bb mice are gray, and bb mice are white. You take a census of the population and record

the following numbers of mice:

Black 1156

Gray 408

White 36

(a) What are the frequencies of the two alleles? (4 pts)

(b) What are the Hardy-Weinberg equilibrium frequencies for these three phenotypes? (4 pts)

(c) A heat wave hits the island. All mice with black fur die from heat stroke, but the other mice survive. What are the new

allele frequencies for the population? (4 pts)

(d) If the population suffers no further cataclysms after the heat wave, and the surviving animals mate randomly, what will

be the frequency of mice with black fur in the next generation? (4 pts)

(e) If the climate is altered permanently, so that mice with black fur die before reproducing, which following statement is

correct? (5 pts)

(1) At Hardy-Weinberg equilibrium, f(B) will equal 0.135.

(2) The fitness of mice with gray fur (ωBb) must be equal to 0.5.

(3) The fitness of mice with black fur (ωBB) is 0.

(4) The B allele will disappear from the population in one generation.

(5) The B allele will disappear from the population in two generations.

6. Which of the following are requirements for evolution by natural selection? Explain your answer. (8 pts)

I Environmental change

II Differential survival and reproduction

III Heritability of phenotypic variation

IV Variation in phenotype

V Sexual reproduction

A) II, III, V

B) II, III, IV

C) I, II, IV

D) III, IV, V

E) II, IV, V

7. Which of the following processes is the source of genetic variation within populations? (8 pts)

A) Reproductive Isolation

B) Mutation

C) Selection

D) Asexual reproduction

E) Genetic drift

Explain your answer including a description of the impact of each on genetic variation.

8. If the population (17,377 in 2018) of folks in Perry, GA, had an f(a) = 0.2 and folks in Valdosta, GA, has a f(a) = 0.6,

then how many people from Valdosta, GA, would have to migrate to Perry to increase the population to

approximately f(a) = 0.3? (5 pts, remember, you can’t have fractional people)

9. What is the Ne of a population with the following annual censuses, [note: the drop in size due to 2010 and 2011 being

extreme drought years]? (5 pts)

2008: 9730

2009: 8810

2010: 3653

2011: 420

2012: 94

2013: 1560

2014: 5650

2015: 8700

2016: 9700

2017: 12110

2018: 15060

2019: 30789

10. Consider the following populations that have the genotypes shown in the following table:

Population AA Aa aa

1 1.0 0.0 0.0

2 0.0 1.0 0.0

3 0.25 0.50 0.25

4 0.25 0.25 0.50

5 0.333 0.333 0.333

6 0.0225 0.255 0.7225

7 0.5929 0.3542 0.0529

8 0.9604 0.0392 0.0004

a. What are p and q for each population? (4 pts)

b. Which of the populations are in Hardy-Weinberg equilibrium? (4 pts)

c. Populations 1 and 2 have a tree fall across their islands so that individuals can cross. If equal numbers of the

individuals occur on each island, what is the new population’s allele frequencies and genotype frequencies

after one generation of random mating? (6 pts)

d. In population 3, the a allele is less fit than the A allele, and the A allele is incompletely dominant. The result

is that AA is perfectly fit (= 1.0), Aa has a fitness of 0.8, and aa has a fitness of 0.6. With no mutation or

migration, graph the allele frequency of the a allele after 10 generations under selection (e.g., Time 0 = q

above, Time 1 = first generation after selection) (8 pts)

e. In population 8, the population size gets radically reduced to 50 individuals, total. What is the most likely

fate of the “a” allele, and what genetic principle would lead you to believe that the case? (4 pts)

11. You digest a linear piece of DNA with two restriction enzymes, BamH1 & Sma1, and get the following sized

fragments (in kb [kilobases]) (10 pts):

BamH1 Xho1 BamH1 & Xho1

10 kb 12 kb 8 kb

6 kb 8 kb 6 kb

4 kb 4 kb

2 kb

Draw the appropriate restriction fragment map based on this data labeling all restriction sites

 
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Cell Biology And Genetics CASE 1!!!!

MN551-1: Integrate knowledge of advanced physiology and pathophysiology across the lifespan with the clinical implications for the advanced practice nurse.

 

ONLY ADDRESS CASE 1!!!!!

Case Study: Cell Biology and Genetics

 

Below are several case studies. Your instructor will either assign you a case study or have you select one. If all of the case studies have not been covered, your instructor may assign a case study that no one has covered. Be sure to integrate your knowledge of advanced physiology and pathophysiology across the lifespan with the clinical implications for the advanced practice nurse.

 

Case Study Posting Requirements

1.    Make sure all of the topics in the case study have been addressed.

2.    Cite at least three sources; journal articles, textbooks, or evidenced-based websites to support the content.

3.    All sources must be within five years.

4.    Do not use .com, Wikipedia, or up-to-date, etc., for your sources.

 

Case Study 1

Marsha and Clement are both carriers of sickle cell disease, a disease that is autosomal recessive. Their first child, Amelia, does not have the disease. Marsha and Clement are planning another pregnancy, but they are concerned about their second child having the condition. Clement’s dad died from complications of sickle cell disease shortly before Amelia was born.

1.    Draw a Punnett square to determine the likelihood of Marsha and Clement having a baby with sickle cell disease. What is the chance the baby will be a carrier of the disease, just like the parents?

2.    Marsha suggested to the nurse at the local family planning clinic that if the baby were a boy he might have a higher risk for developing the disease, just like his grandfather. If you were this Practitioner, how would you respond?

3.    When Amelia, who does not have sickle cell disease, grows up and marries someone who does have the disease, how likely is it that her children will have the disease?

Case Study 2

Maria is a sedentary, 68-year-old woman who is overweight. She complains that her hands and feet are always cold, and she tires quickly when cleaning the house. At her most recent visit to her doctor, her blood pressure was 184/98 mm Hg. She has edema around her ankles and legs, and her physician is concerned about an echocardiogram that indicates Maria has an enlarged heart.

1.    Identify two reasons why Maria will have tissue ischemia. How might this lead to hypoxia?

2.    What two early and reversible changes occur to tissue cells when they are hypoxic?

3.     What specific type of cellular adaptation has taken place in Maria’s enlarged heart? What made you come to this conclusion?

4.    Predict why Maria’s heart has become enlarged. Why doesn’t this enlargement give her the same cardiac strength and endurance as a well-trained athlete?

Case Study 3

Kevin worked for 10 years at a uranium mine, excavating uranium for a nearby nuclear power plant. Now, 25 years later, he has small cell lung cancer. Kevin is anorexic and has lost a considerable amount of weight. His muscles are wasting, and he is weak. He tries to move around the house throughout the day but tires easily. It has been difficult for him to access care, and the treatment for his cancer is just starting.

1.    With the ongoing exposure to the ionizing radiation, DNA damage occurred. Outline the three stages of carcinogenesis that occurred after his exposure to radiation.

2.    Kevin is normally a fit and active man, and his wife often commented on how much food he used to eat after a day at mine. Why would there be muscle wasting and weight loss now? Explain your answer using your

3.    knowledge of the metabolic changes seen with cancer.

4.     In some cancer patients, muscle weakness may result from the production of onconeural antigens. Describe the effects of these antigens. What form would this process likely take in Kevin’s situation?

Case Study 4

Felicity is a very busy 29-year-old woman in a professional career. She has diabetes mellitus, and is also pregnant for the first time. Due to her busy schedule, it took her three weeks to visit the family doctor to have the pregnancy confirmed. Felicity became very concerned when her physician asked whether she had been taking folic acid. It was all Felicity could do to remember to manage her insulin levels, and taking folic acid supplements was something she hadn’t even considered. Her doctor told her to take 600 μg of folic acid daily and advised Felicity to return later for maternal serum marker testing.

1.    Explain the potential teratogenic effect of folic acid deficiency on the developing fetus. What other risk factor is noteworthy in Felicity’s case?

2.    What is the benefit of maternal serum marker testing? What other test would be particularly useful to monitor the development of Felicity’s baby in this situation?

 

3.    When is the fetus most vulnerable to the effects of teratogens and why?

Assignment Requirements:

Before finalizing your work, you should:

·         Ensure you have written at least four double-spaced pages.

·         be sure to read the Assignment description carefully (as displayed above);

·         consult the Grading Rubric (under the Course Home) to make sure you have included everything necessary; and

·         utilize spelling and grammar check to minimize errors.

·         follow the conventions of Standard American English (correct grammar, punctuation, etc.);

·         be well ordered, logical, and unified, as well as original and insightful;

·         display superior content, organization, style, and mechanics; and

·         use APA 6th Edition format as outlined in the APA Progression Ladder.

 
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Bio 101 Db Forum 1

can someone do this for me for tomorrow please

 

Discussion Board Forum 1

 

Topic: What Should You Eat?

 

You probably want to live a long and healthy life on this earth. What  are you willing to do to make that possible? Here is an assignment that  can improve the quality of what you eat, and hence, the quality of your  life. Let’s develop the rudiments of a maintenance diet for you—a desirable, workable, realistic, non-faddish maintenance diet—one you follow permanently. You have several reference sources:
– the Bible’s many prescriptive texts regarding nutrition (ignore “descriptive” texts)

– your textbook’s chapter on biomolecules; how they are built and used

– the course Presentation entitled “Biomolecules and Nutrition”

– trustworthy sources such as

– USDA MyPlate:  https://www.choosemyplate.gov/MyPlate-Daily-Checklist

– the Mayo Clinic website.  http://www.mayoclinic.org/healthy-lifestyle

– Very Well website (David Katz’ site):  https://www.verywell.com/

– Web MD:  http://www.webmd.com/diet/default.htm

The foods you select will contain the same classes of biomolecules that  you read about in your textbook: carbohydrates, lipids, proteins,  nucleic acids, vitamins, and minerals.  Use the following procedure to  build your diet.
For your Thread:
1.  Click on the link below.  This will give you a Word document “work  table” to add your foods to.  You may wish to print a copy for research  purposes.

What Should I Eat – Work Table.docx   Click for more options

2.  Go to the “My Plate Check List Calculator https://www.choosemyplate.gov/MyPlate-Daily-Checklist-input   Enter your data to determine how many calories your daily diet should contain. Record this number in your work table.
3.  Now go to the USDA MyPlate web site: https://www.choosemyplate.gov/MyPlate-Daily-Checklist  In  the Table, about the middle of the page click on the box representing  your caloric needs.  Some serving amount data will pop up allocated into  5 food groups.
4. Record the suggested serving amounts in your  work table to give quantitative values to the categories you will build  your diet around.

“What Should I Eat?” – Work Table:

 

 

Total Calories:

/per day

 

 

Food Group:

Biomolecules in this Group in Order of Relative Biomass*

Serving Amount:

My Food Choices:

 

 

10 Fruits

Carbs, Proteins, Oils

My first food, my second food, my third food, my fourth food, etc.

 

 

12 Vegetables

Carbs, Proteins, Oils

 

 

12 Grains

Carbs, Proteins, Oils

 

 

9 Proteins

Proteins, Fats, Oils

 

 

7 Dairy Items

Depends on the Food

 

*as a generalization

5.  Start to choose foods using the guideline comments given on the webpage.   Use other web pages listed above to get commentary on foods you think  (!) are healthy for you.  Here are two useful ones to give you ideas:

https://www.verywell.com/macronutrients-made-simple-4128991 , https://www.verywell.com/the-basics-of-a-healthy-balanced-diet-2506675

6.   Delete the sample foods listed in the “Fruits” category.  They are  there to show you the format for your own additions.  List specific  foods that you would eat for each group according the numbers indicated  in the first column. The second column of the table will help you with  your category accuracy.  Do not reuse any food under a second category.  You will thus select 50 foods for your diet—not a huge variety, but it’s  a start!  “Leafy greens” or “seafood” are food categories, not   specific foods.
7.  “Mouse over the upper left-hand corner of  the table to find the tiny navigation box and click on it.  This  highlights (“selects”) the whole table.  Copy it to your computer’s  “clipboard”.  You can now paste this table into the “Message Box” of  your open “Thread” in the discussion board.  Along with your table, you  may submit up to two prescriptive Bible passages that you feel most  constrain your dietary thinking.

Hints:

•     Your goal is always to improve your own list. Please include about 70  words for your diet (50 foods, some hyphenated as needed) with space  left over for two Bible verses for a total of 120 words.  That’s your limit!

•    Foods differ in their density of a wide variety of nutrients as  compared to just the calories they give you.  Which sweet would be  better for you:  hard candy or a fig bar?
•    What is in the food item you’ve selected? Check out:  http://ndb.nal.usda.gov/  to find out.
•    Suppose Mayo Clinic wisdom and Biblical wisdom seem to conflict.  Which source will you defer to and why?

 
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Man Vs. Nature

C:\Users\jody.evans\Pictures\GCU Logo.jpg

Man vs. Nature

This week we are discussing Genetically Modified Foods and just like any product available there are pros and cons. This will be a hands-on assignment that will require you visit a grocery store to compare and contrast Genetically Modified and Organic produce. Be sure to select the same type of fruit or vegetable to compare and contrast. You are not required to buy the product!

Note: You may ask a store employee for help to identify which produce is organic and which is a Non-organic.

Student name:

What fruit or vegetable are you comparing?

  Non-organic Organic
Price    
Characteristics:

· Color

· Size

· Smell

· Weight

   
Where was it grown/imported from (country, state, etc.)?

 

 

 

   
Where were observations made (farmers market, Walmart, etc.)?

 

 

 

 

   

Short Answer Questions (Minimum of 150 words each)

1. What are the some of the arguments for labeling GM foods? What are some of the arguments against it?  What side do you feel is more valid, why?  Provide at least one source per argument (one for and one against) to support your claims.

 

 

 

2. Do you feel as though GMO foods are the answer to end world hunger? Why or why not? If GMOs are the answer to the shortages, why do food prices keep going up?

 

 

 

 

 

3. What are some ethical concerns or benefits of using GMOs based on the Christian Worldview? (Refer to “Here’s What Religious Experts Have to Say About Faith and GMOs” for help answering this question.)

© 2016. Grand Canyon University. All Rights Reserved.

 
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Acids And Bases Lab

[Write Name Here]

Professor Meyer

Acids and Bases Lab

Due: Friday September 25th, 2020

 

Acids and Bases Lab

 

Substance pH Paper Value pH Meter Value
1 – Cal 4.0 pH    
2 – Cal 7.0 pH    
3 – Cal 10 pH    
4 – QC high    
5 – QC low    
6 – Urine #3    

—————————— Check Your Understanding —————————

 

1.1 Carbon dioxide is bubbled through a pink basic solution and a color change occurs. What color is seen and what compound is formed?

 

 

1.2 Which of the two methods to measure the pH of a sample is more accurate: pH paper or pH meter?

 

 

1.3 The commonly used indicator phenolphthalein will turn [fill in the blank here] in a basic solution and in an acidic solution is [fill in the blank here]

 

2.1 What causes heartburn and how do over-the-counter antacids work to help people suffering with heartburn?

 

———————————— Chapter 4 Review —————————————

 

1. Compare and contrast an acid and a base, and provide examples of each

 

 

2. The pH scale has a range from 0 to [fill in the blank here]. A substance with a pH less than 7 is considered a/an [fill in the blank here] and a substance with a pH greater than 7 is a/an [fill in the blank here].

 

 

3. If tomato juice has a pH of 3, how many more H+ ions are present in lemon juice with a pH of 2?

 

 

4. Which of the following reactions are seen with an acid?

a. An acid turns blue litmus paper red, is colorless in phenolphthalein, and turns methyl orange indicator yellow.

b. An acid turns blue litmus paper red, is colorless in phenolphthalein, and turns methyl orange indicator red.

c. An acid turns blue litmus paper blue, turns phenolphthalein pink, and turns methyl orange indicator yellow.

d. An acid turns blue litmus paper blue, is colorless in phenolphthalein, and turns methyl orange indicator yellow.

 

5. One method used to determine the pH of a substance is using pH paper. Describes the composition of the paper, how the procedure is performed, and the accuracy compared to a pH meter.

 

6. What is an ionic bond? Give an example.

 

7. What is a covalent bond? Give an example.

 

8. Label each characteristic or example below as describing either acids (A) or bases (B):

 

Characteristic Listed   Acids (A) or Bases (B)
These have a bitter taste.    
These contribute one or more hydrogen atoms to a solution when it dissociates in water.    
These have a sour taste.    
These have a pH greater than 7.    
These react with certain metals, which results I liberation of hydrogen gas.    
These have a slippery feel.    
The chemical compound delivered by a be sting is an example.    
These release hydroxide ions in solution.    
Carbonated water is an example.    
Sodium hydroxide is an example.    

9. An old saying is “oil and water don’t mix.” This is the same situation for oil and vinegar. Explain why?

 
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Lakota Ethnobotany Curriculum With Richard T. Sherman

Increasing Access to Business Education Opportunities

ETHNOBOTANY of the NORTHERN PLAINS TRIBES

As told by Oglala Lakota, Richard T. Sherman

 

 

Upo! Welcome!Upo! We welcome you to enjoy this workbook as an accom-paniment to our video series: Ethnobotany of the Northern Plains Tribes as told by Oglala Lakota, Richard T. Sherman. This workbook provides viewers with additional information about ethnobotany of the Northern Plains. Each segment of this workbook corresponds with a segment of the video and can be used to enrich your learning experience.

ABOUT OUR INSTRUCTOR Richard T. Sherman is an elder from Kyle, South Dakota. Richard grew up on the Pine Ridge Indian Reservation, which is home to the Oglala Lakota Oyáte (nation). He has practiced subsistence hunting and gathering for over six decades. His philosophy of wildlife and use of native plants stem from the traditional education he learned while listening to his Lakota elders, and from western scientific methodologies. He studied wildlife management at Utah State University, and graduated with a Masters in Regional Planning from the University of Massachusetts, Amherst.

Richard established the first comprehensive fish and wildlife code for the Oglala Sioux Tribe. In 1985 he introduced the Indigenous Stewardship Model as an alternative to western natural resource management. Currently, he conducts guided ethnobotany tours and day hikes in Badlands National Park, Wind Cave National Park, and the Black Hills National Forest. Richard also provides wild game for people who are in need, both on and off the Reservation.

 

 

Ethnobotany of the Northern Plains Tribes

1

Table of Contents Quick Start Guide 2 Introduction: Opening 3 Lesson 1: Food Plants 7 Lesson 2: Medicinal Plants 13 Lesson 3: Ceremonial Plants 18 Lesson 4: Poisonous or Harmful Plants 22 Lesson 5: Sustainability & Plants

27 End Notes 29 Additional Resources 30 Appendix A: Food for Thought Recipes 31 Appendix B: Gathering More Data 34 Appendix C: Plants List 36

 

 

Quick Start Guide The Ethnobotany of the Northern Plains Tribes course is comprised of an introduction and five lessons to provide you with knowledge on traditional Lakota uses for plants indigenous to the Northern Plains region.

• Introduction: Opening

• Lesson 1: Food Plants

• Lesson 2: Medicinal Plants

• Lesson 3: Ceremonial Plants

• Lesson 4: Poisonous or Harmful Plants

• Lesson 5: Sustainability & Plants

LESSON COMPONENTS Each lesson has been designed with a variety of components to help maximize your learning experience. Lesson components are indicated throughout this workbook by the icons described below.

2

 

 

Ethnobotany of the Northern Plains Tribes

WATCH Refer to your DVD or the website address noted in the workbook to watch each les- son video.

EXTRA FUN In your spare time, try a more in-depth activity to deepen your personal understanding of the course material.

ACTIVITY Complete the activity in the workbook to reiterate the in- formation covered in the video.

INTERESTING INFO Take time to read these inter- esting points of information to gain a better understanding of the topic at hand.

QUIZ Take a short quiz to see how much you have learned after watching the video and com- pleting the activities.

SHARE Let us know how your learning experience went. Share your thoughts on our Facebook page or give us a call.

 

 

Opening Video

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The Pine Ridge Reservation This ethnobotanical tour takes place in southwest South Dakota on the Pine Ridge Indian Reservation in the South Buffalo Pasture of Allen, along the Medicine Root Creek north of Kyle, and at the sacred site known by many northern plains tribes as Bear Butte, near Sturgis. The filming

Ethno, by the way, basically means culture and botany means plant. So, an ethnobotanist studies plants and their usefulness for a particular culture. Though many people today would consider Richard an expert, in the past, Plains tribes needed to know how plants could be used by their people for food sources as well as medicinal and ceremonial purposes. Still today, the Lakota spend one to three hours a week gathering plants!1

Since the Treaty of 1868, southwest South Dakota has been considered home to the Oglala band of Lakota. The Lakota once utilized lands for subsistence living that stretched north of the Canadian

WHAT IS ETHNOBOTANY? “Ethno” means culture. “Botany” means plant. An ethnobotanist studies plants and their usefulness for a particular culture.

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INTRODUCTION

 

 

occurred during the month of June. The growing season for harvestable plants in southwest South Dakota is from April to October. border, south of the Platte River in current Nebraska, west to the Rocky Mountains of Colorado, Wyoming, and Montana, and east to the Missouri River as it flows through St. Louis. The Lakota and many other tribes, despite not being able to hunt, gather, trade, and move freely as in the past, still value their traditional teaching; honor the wisdom of their elders; and practice the ways of their culture through dance, art, language, prayer, ceremonies, hunting, and gathering.

MAP OF PINE RIDGE RESERVATION: HOME OF THE OGLALA LAKOTA

Map artwork created by David Bartecchi of Village Earth.

With over 2 million acres, the Reservation is comprised of mixed grass prairie, along with the occasional creek (wakpála) or river (wakpá) providing water (mní) to the wildlife; canyons and woodlands with fresh springs and cottonwood tree (wá ačhaŋ) groves; along with wetlands and the ǧ South Unit of the Badlands National Park. The Badlands National Park hosts deer, antelope, buffalo, and bighorn sheep as well as predators. Historically, the Reservation was home to prominent figures such as Chief American Horse, Chief Red Cloud, spiritual leader Black Elk, and Olympic Gold Medalist Billy Mills.

Quiz After watching the Opening video segment and reading the section above, select the best response for each question.

Ethnobotany of the Northern Plains Tribes

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1. Pine Ridge Reservation is home to: A. the Oglala Lakota or Oglala Oyáte B. the Badlands National Park, South Unit C. Chief Seattle’s descendants D. Both A and B

2. Richard Sherman is an: A. Oglala Lakota B. Elected tribal councilman C. Ethnobotanist D. Both A and C

3. Richard’s information comes from _________________ and ____________________. A. traditional education of Lakota elders; the western scientific method B. religious beliefs; the Sherman thiyóšpaye (extended family) C. the National Park Service; Lakota Funds D. All of the above 4. This video series will cover plants that are useful for: A. Food B. Medicine C. Ceremonies D. All of the above

5. On average, Lakota households spend 1 to 3 hours a ________ gathering plants. A. Year B. Day C. Month D. Week

6. The growing season for harvestable plants in southwest South Dakota is: A. February to September B. April to October C. January to September

D. March to November

7. During the World War II period on the Reservation: A. Hunting was temporarily banned B. There was greater plant variety C. The landscape looked a lot healthier D. Both B and C

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INTRODUCTION

DO YOU GATHER PLANTS? Wow, think about that! In an entire year, how many hours does your family spend gathering plants?

 

 

The Pine Ridge Reservation is a vast area full of useful plants that grow naturally.

Ethnobotany of the Northern Plains Tribes

ON THE HUNT FOR EDIBLE PLANTSTake two hours out of your day and try to find local edible plants around your home. If you are unsure of which plants are edible, go to your local library and pick out some books, search online, or contact an expert to help you identify others that may be useful.

Knowledge is brain food!

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Food Plants Video

Lakota Food The Lakota word for food is wóyute. The foods featured in the video provide just one small sample of traditional and contemporary Lakota foods. In traditional times, the Oglala Oyáte (nation) traded foods and goods, such as their main source of carbohydrates— thíŋpsiŋla (wild turnip)—and buffalo hides, to other nations. Thíŋpsiŋla was peeled, braided, and dried so it would be easy storage or light cargo on long trips for trade or for moving camps.

lakotafunds.org/learningcenter/lesson/food-plants/

Some of their trading partners were the tribes along the Mis- souri River Valley such as the Mandan and Hidatsa farmers to the north but also their cousins, the MnikhAówožu, whose name translates to “Planters by the Water.” They also traded with their Dakota relatives, the Bdewákhaŋthuŋwaŋ or Mdewakan- ton (Dwellers by the Sacred Lake), who grew corn and beans on the Minnesota and Missouri River; and their Nakota relatives, the IháŋkthAuŋwaŋ or Yankton Dwellers at the end of the Camp Circle), of eastern North and South Dakota. According to an 1803 (

witness, the IháŋkthAuŋwaŋ were “tillers of the soil.”2

LAKOTA LANGUAGE LESSON The Lakota word for food is wóyute.

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LESSON 1

 

 

A long thíŋpsiŋla braid hanging from a cottonwood tree.

Gathering Data Today, Lakota tribal members eat food grown by nations from all over the world. However, there have been recent efforts across Pine Ridge to start community gardens, family gardens, and a farmers’ markets to enhance the production of healthy local food. Research shows nearly 59% of the Oglala ate a traditional Lakota food in the last month.3

As in the past, waháŋpi or soup, is a favorite meal to many Lakota families. One waháŋpi in particular is known by Spanish speakers throughout North America as menudo, or beef tripe soup. The Lakota term is t aní a. Today, Lakota use buffalo tripe, but beef tripe has become a popular ȟ ǧ substitute. Dried corn (ẃastunkala) is often added to t aní a. In times past, the dried corn was ȟ ǧ acquired from northern trade partners. Several of the foods and other plants found in this video series make good compliments, flavor enhancers, and thickeners for waháŋpi. While watching the video, fill out the chart on the following page.

Data is the scientific term for information used to tell a story. Collecting data is often referred to as “gathering” data. Just as Lakota have always hunted and gathered food, there are many Lakota biologists, ethnobotanists, astronomers, and other scientists who gather data. This exercise will allow you to collect, or “gather,” data about gathering food. The data you record below will help you gather your own plants in the future.

LIFE-SAVING PLANT KNOWLEDGE Could you imagine a group of people speaking a language you don’t understand providing you with knowledge to save your life. Just like the Mormons have the Ute to be thankful for, we have so many other groups of people to be thankful for as well!

Ethnobotany of the Northern Plains Tribes8Ethnobotany of the Northern Plains Tribes Ethnobotany

of the Northern Plains Tribes PHOTO LAKO TA NAME COMMON NAME SCIENTIFIC NAME PHYSICAL PROPERTIES USES Gathering Data Complete this chart while watching the video on food plants.

foodteaFruit puddingTurnip for food

Similar to wild onion but has a lily up top

Tall purple flowerShiny leaves with cherries coming out of the bottom

Fuzzy plant

Calochortus gunnisonii

Amorpha canescensPrunus PumilaPsoralea esculenta

Sego lilyLead plantSand cherryPrairie turnip

Psin thankazintkathachanaunyeyapithinpsinlaptethawote

Ground plum

Astragalus crassicarpus

Small green and red balls

Eat them raw or use in cooking

psinsicamna

Wild Onion

Allium canadense

It has a small onion at the bottom

soup

 

 

Quiz After watching the Foods video segment, reading the section above, and completing the activities, select the best response for each question.

1. The main thing to realize when you collect plants is: A. Meat eaters and vegetarians have negative impacts on the environment. B. They will always be abundant. C. Everything you do has an impact, so we need to be as kind to the land as possible. D. You are what you eat.

2. Death Camas can easily be mistaken for _________________, because the flower looks similar. A. Medicine B. Wild Onion C. Thíŋpsiŋla D. T at áŋka t aló ȟ ȟ ȟ

3. ___________________ is a soup made of buffalo or beef tripe. A. Medicine B. Death Camas C. Thíŋpsiŋla D. T aní a ȟ ǧ

4. Research shows nearly ___ of the Oglala ate a traditional Lakota food in the last month. A. 75% B. 20% C. 59% D. 100%

5. Thíŋpsiŋla is peeled, braided, and dried. Traditionally, it was the main source of _____________ for the Oglala.

A. carbohydrates B. protein C. fiber D. Vitamin C

6. _______________ and _________________ are both good for making wóžapi. A. Choke Cherries; Sand Cherries

PH OT

O

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B. Sand Cherries; Lead Plant C. Sego Lilies; Choke Cherries D. Choke Cherries; Wild Onion

7. The ________________ literally kept the Mormons from starving to death. Thus, the Sego Lily is now the state flower of Utah.

A. pšiŋšičamna B. ptet áwoteȟ C. pšiŋ t áŋkaȟ D. ziŋtkát ačhaŋȟ

 

 

Ethnobotany of the Northern Plains Tribes

The Ground Plum is part of the legume family. The Sego Lily has a beautiful flower and an edible bulb.

“GATHERING” MORE DATAThere is another blank Gathering Data chart (similar to the one you filled out during the video) in the Appendix B at the back of this workbook. This chart can be used for you to gather your own data related to food plants that grow near your home.

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LESSON 1

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Medicinal Plants Video

Lakota Medicine The Lakota word for medicine is p ežúta. According to Lakota stories, mní, or water is known as the first ȟ medicine. Water is life. Of course, water is not the only natural element used for healing and medicinal purposes. Many pharmaceutical drugs for example, were derived from native plants.

FOOD FOR THOUGHT: HEALTHY CONTEMPORARY RECIPES THAT INCLUDE

tognake (generosity) is a Lakota value that runs through all aspects of Lakota life and culture. One way to show generosity is through the provision of food. Most Lakota

gatherings

History is also extremely important to the Lakota. History connects Lakota ancestors to the present and future. In Lakota culture, history is taught through the telling of stories. A more contemporary way to tell a story is by sharing recipes that have been passed down from generation to generation. Recipes communicate a story of love, skills, and prayer which is passed down in the form of a meal. Just like different peoples and their cultures, recipes change over time. Try one of these modern recipes located in the back of the workbook that are made with

Buffalo Burger Deluxe with Roasted Potatoes

Buffalo Dumpling SoupPrairie Power CocktailDelicious Three Sisters

SHARE A LAKOTA FOOD SELFIEWe know you are going to prepare an awesome Lakota meal! Share a photo of your Lakota meal on our Facebook page at facebook.com/ LakotaFundsCDFI.

lakotafunds.org/learningcenter/lesson/medicinal-plants/

Ethnobotany of the Northern Plains Tribes

60,000 years ago!4

The timeless tradition of herbal medicine remains strong today. This is reflected by the increasing presence of herbs in local food stores and a resurgence of growing herbs and native plants for medi- cine. According to the World Health Organization, 75% of the world’s populations are using herbs for basic health care needs.5

LAKOTA CULTURE LESSON The Lakota believe water is the first medicine.

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LESSON 2

 

 

Salicin, the pain killer found in Aspirin as noted by our instructor, Richard, is found in the cottonwood and all other members of the willow family. Native people have known the healing power of plants for thousands of years. In fact, in what is now modern day Iraq, herbal medicine has been traced back to

Yarrow is a plant found all over the world, and used for The resin from flowering heads at the top of the Curlythe same medicinal purposes across cultures.cup Gumweed plant cures poison ivy.

The wild rose, or uŋžiŋžiŋtka, grows abundantly on the South Dakota prairie.

The wild rose is one of the many plants used for common ailments. After removing the seeds, the flesh of the berries, or hips, are used as food or a beverage. The root bark is used to make tea for treating diarrhea. Even in 2015 diarrhea was a leading killer of children, accounting for 9% of all deaths among children

Making ConnectionsOn the following page, draw lines to connect the Lakota name and the medical uses to the correct picture. One is already completed as an example.

14

 

 

under age 5 worldwide. This equals over 1,400 young children dying each day, despite the availability of a simple treatment.6

Ethnobotany of the Northern Plains Tribes

Native and Western: Making Connections

Following the example, draw lines to connect the Lakota name and the medical uses to the correct picture.

 

 

LAKOTA NAME PHOTO & COMMON NAME MEDICINAL USES

heyókhAa thAaphAéžuta

Scarlet Globemallow

Asthma relief and poison ivy treatment

čhaŋšíŋšiŋla

Wild Rose

A styptic: used to stop bleeding

thíčaničahu

Yarrow

Treats skin ailments from burns, swellings, and rashes

hAaŋté čhaŋhAlógUaŋ

Slimflower Scurfpea

Treats diarrhea and used in drops to treat eye inflammation

uŋžiŋžiŋtka

Curlycup Gumweed

Pain reliever, similar to aspirin

 

 

Quiz After watching the Medicinal Plants video segment, reading the section above, and completing the activities, fill in the blanks below with choices from the word bank. A word

can only be used once. Some words will not be used at all.

1. The Lakota word for medicine is Phezuta______________.

2. Water__________ is known as the first medicine.

3. The Lakota name, heyók a t ap éžuta, refers to heyók a medicine. It was used during ȟ ȟ ȟ ȟ ceremonies to pull meat out of boiling water without getting burned. The common name for this plant is Scarlet globemallow____________________.

4. The Lakota root word for Curlycup Gumweed, čhaŋšíŋ, is the same word used for gum___________ today.

5. Yarrow_______________ is the common name for the plant used by some Sundancers to heal wounds from piercings.

6. Slimflower scurfpea root can be used to make a tea. It’s medicinal properties are similar to Wild Rose____________.

7. The scientific name for Yarrow is Achillea millefolium_______________________. Millefolium_______________ means “a thousand leaves” referring to the intricate leaf structure.

iníka Aǧ Achillea millefolium lúta T uŋkášilaȟ p ežútaȟ tenuiflora

wóyute (food) t até (wind)ȟ t at áŋka (buffalo)ȟ ȟ Scarlet Globemallow gum Aspirin

mní Millefolium Prunus pumila pumila Psoralia tenuiflora Yarrow

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WORD BANK

 

 

NATURAL MEDICINEDuring this week, take time to find one plant, or natural medicine in a store made from a plant, and begin using it for an ailment (ie. skin rash) or symptom (ie. low Iron). Keep record of the results and let us know what you find.

SHARE YOUR NATURAL CURESIf you use plants or herbs as natural medicine, let us know. Share your natural cures with us on our Facebook page at facebook.com/ LakotaFundsCDFI.

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LESSON 2

 

 

Ceremonial Plants Video

Lakota Ceremonies Ceremonies and rituals are an important part of any culture. This is especially true for the Lakota, who value spirituality as part of everyday life. The plants in this video segment can be used for a variety of purposes, including ceremonial use.

There are special locations used by the Lakota for praying, vision quests, and other ceremonies. Bear Butte near Sturgis, South Dakota is one of those places. It is considered sacred to the Lakota and many other plains tribes as well. Areas such as Bear Butte are continually threatened by development,

recreation, and other activities that have negative environmental impact on those areas. Help keep sacred sites sacred by

lakotafunds.org/learningcenter/lesson/ceremonial-plants/

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Ethnobotany of the Northern Plains Tribes

 

 

honoring the earth and respecting Indigenous peoples’ spiritual practices.

For over a century, Lakota people and other Native Americans were banned from expressing themselves spiritually. This included participating in ceremonies. It was not until the passing LEGALITY OF LAKOTA CULTURE In the past, participation in

Lakota and other Native American ceremonies was illegal. One of the most notorius spiritual practices was the Ghost Dance, which came to a head near Cuny Table on the Pine Ridge Reservation.

of The American Indian Religious Freedom Act of 1978 and the Religious Freedom Restoration Act of 1993 that Native peoples could freely practice their beliefs again. Today, Sundance ceremonies, sweat lodges, and vision quests occur all over the world. In fact, some hospitals, rehabilitation programs, and correctional facilities utilize the sweat lodge as a form of healing and rehabilitation for praying and purification.

Lakota ceremonies often incorporate tobacco, sometimes mixed with other plants. Red Willow is an important part of these mixtures. Red Willow is actually not a willow at all, but it is technically a Red Osier Dogwood. The leaves of the Red Willow are not smoked. The inner bark of the small tree is scraped and smoked.

Šá is the Lakota word for red. The thin branches on the Red Willow are red, which is where the word was derived.

In addition to Skunk Bush, mint, or čheyáka, is often added to tobacco mixtures. Mint can also be used to make tea and stored in dry food goods to keep insects away.

Creating a Tobacco Mixture Tobacco is a very important part of Lakota ceremony. If you were asked to collect plants for a tobacco mixture, which plants would you collect?

Look in the left hand column, and then circle all of the plants you would collect. Then draw a line from the circled choices, connecting them to their Lakota name.

LAKOTA LANGUAGE Čh

a ŋ is the Lakota word

for tree.

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LESSON 3

 

 

MATCHING FOR YOUR MIXTURE

Men’s Sage Skunk Bush Red Willow Silver Sage Bearberry Lead Plant

čhaŋšáša čhaŋúŋkčemna p eží ota t ot ó ȟ ȟ ȟ ȟ wá ačhaŋ ǧ čhaŋlí wa péȟ ziŋtkát ačhaŋȟ

Ethnobotany of the Northern Plains Tribes

 

 

Quiz After watching the Ceremonial Plants video segment, reading the section above, and

completing the activities, answer True or False to the following statements. 1. Sacred sites such as Bear Butte are continually threatened by development and recreation. True False

2. The cottonwood tree, or wá ačhaŋ, is used as the sacred pole for the Sundance. ǧ True False

3. During ceremonies, sweet grass brings in good spirits. True False 4. Silver Sage, Women’s

Sage, and Men’s Sage are of the same family as the Sunflower. True False 5. Sages used for ceremonies are of the same family as sage used in Thanksgiving dressing. True False

6. Skunk Bush is of the peanut family. True False

7. Native Americans could not freely practice their ceremonies until 1993. True False

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CONTEMPLATING YOUR OWN CEREMONIESFor the next several days, think about the ceremonies and rituals that you practice or believe in. Are they things you do on a daily basis? Weekly? Monthly? Yearly? Which plants or foods are involved in the ceremonies that you participate in? Do you feel comfortable and accepted while practicing your beliefs? Could you imagine your ceremonies being banned, or being punished for practicing ceremonies that are important to you or your family?

Ethnobotany of the Northern Plains Tribes

 

 

Bear Butte near Sturgis, South Dakota is considered a Skunk Bush is commonly used in tobacco mixtures for sacred place by the Lakota. Lakota ceremonial purposes.

Poisonous & Harmful Plants Video

lakotafunds.org/learningcenter/lesson/poisonous-and-harmful-plants/

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LESSON 3

Starting in the 1400s, Native tribes throughout the country were fighting back against European colonists. Juan Ponce de León, early Spanish explorer and conquistador, was wounded by a poison arrow which was shot by a Native warrior in the area of present day Florida. The conquistador later died from the wounds.7

REMEMBERING HARMFUL PLANTS To protect yourself from poisonous plants, consider memorizing a mnemonic device. A mnemonic device is something that can be used to assist in memorizing the features or definition of a certain word or object.

POISON WARFARE Native Americans used poison arrows in battles with European colonists in the 1400s.

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Poison on the Prairie As the saying goes, “One man’s pleasure is another man’s poison.” Poison has played a sinister role in different aspects of world history. The ancient Greek philosopher Socrates drank hemlock as a death punishment in 400 BC. Some less potent forms of poison have probably left a mark on your legs or arms after a hike.

Ethnobotany of the Northern Plains Tribes

 

 

For example, people often confuse Poison Ivy with Virginia Creeper, which has five leaves. With Poison Ivy, you can remember:

“Leaves of three, let it be.”

Water Hemlock, a close relative to the Poison Hemlock featured in the video, has leaf veins that connect from the central vein of the leaf to the inner notches (or cuts) of the jagged edges of the leaf. So if you are near a creek and you see a tall plant with small white flowers and leaves shaped like a spear head with jagged edges, just remember:

“Vein to cut, pain in the gut.” DEADLY POISON: HEMLOCK

Poison Hemlock can be recognized by the purple spots on its stem and small white flowers.

Plant: Malign poison ivy_____________________

Mnemonic Device: Berries white, run in fright______________________

________________________________________________________

YOUR MNEMONIC DEVICE

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LESSON 4

 

 

Creating a Memory Tool Create your own mnemonic device to protect you from a poisonous or harmful plant. Or create your own mnemonic device to help you identify a useful plant. Share the saying with

others.

Finding a Cure Although poisonous and harmful plants grow naturally in the Northern Plains, so do their antidotes. There are many plants that have medicinal abilities to heal some of the harm other

plants can cause.

We learned in this lesson’s video that Poison Ivy can cause a skin rash, but the Medicinal Plants video taught us that Curlycup Gumweed is used as treatment for Poison Ivy. Below is a large image of Poison Ivy, and below it are four images of other plants found on in the Northern Plains.

Connect the image of Poison Ivy to the image of Curlycup Gumweed.

Poison Ivy, recognized by its “leaves of three,” can cause an itchy rash. One of the plants below can heal this rash.

WHICH ONE IS CURLYCUP GUMWEED?

Quiz After watching the Poisonous & Harmful Plants video segment, reading the section above, and completing the activities, select the best response for each question.

1. The Snowberry plant is not poisonous, but________________________________________. A. ticks are frequently found on them, so beware.

B. it attracts poisonous snakes. C. it helps treat skin ailments from poisonous plants. D. it looks similar to a poisonous plant.

2. How can you tell the difference between Death Camas and Wild Onion? A. Death Camas has

three leaves.

Ethnobotany of the Northern Plains Tribes

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This one

 

 

B. Death Camas does not smell like an onion. C. They grow in different environments. D. Their bulbs are similar.

3. Poison Ivy belongs to what plant family? A. Nightshade B. Lettuce C. Cashew D. Legume

4. Poison Hemlock grows tall, has a hollow green stem, grows small white flowers, and ________________________.

A. is a good fire starter B. has purple spots along the stem. C. is of the carrot/parsley family. D. Both B and C 5. Stinging Nettle _______________________________________. A. contains about 25% protein

at peak growth. B. has little spikes along the edges of the leaves that creates an itching sensation. C. can be lashed onto areas with arthritis to relieve symptoms. D. All of the above.

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LESSON 4

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THE GOOD AND THE BAD OF STINGING NETTLEStinging Nettle is native to four continents and has been introduced to many more continents over time. Try to find a Stinging Nettle plant where you live. Be careful touching it, as it will create a burning and itching sensation for several minutes. Cut up some of the leaves and put them into a soup. They add flavor, color, vitamins, and protein, and the cooking process renders them edible. Stinging Nettle becomes completely harmless!

 

 

Ethnobotany of the Northern Plains Tribes

SHARE THE STING Were you able to find a Stinging Nettle plant in your area? Share a photo of your Stinging Nettle harvest on our Facebook page at facebook.com/ LakotaFundsCDFI.

ALLERGIES BE GONE! The Lakota make a tea with dried leaves of the Stinging Nettle plant to treat allergies.

 

 

Sustainability Video

Sustaining Plant Life Food, medicine, rituals of life, and even elements that sometimes seem harmful are necessary for all of us to move forward in a good way. Everything we do as humans has an impact on everything else. Just by entering a room, we change the molecular make-up of that room, forever. Just a smile, can change someone’s entire outlook. It’s like a domino effect.

lakotafunds.org/learningcenter/lesson/sustainability/

If we do something that is not right with nature, it will come back to us at some point. So, as much as we can, we need to care for nature because nature takes care of us. “It’s not so hard”, according to Richard, “when you try to match your heartbeat with that of the land.”8

When you are harvesting plants for your own use, it is important to make sure there is enough left to keep growing for next season. All plants have built-in mechanisms for reproducing. By knowing them, you can be sure to respect the ways of nature.

LAKOTA LIFESTYLE Over 40% of the Oglala Lakota ate a wild plant food within the last month.

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LESSON 5

 

 

Symbol of Sustainability Use the box below to draw a symbol. Draw a symbol to remind you, and to help you explain to others, that everything is connected.

Use this symbol as a reminder of how as humans, we have an important role in taking care of nature that makes life possible for all of humanity. Cut the symbol out so you can carry it with you, or show to others.

Ethnobotany of the Northern Plains Tribes

YOUR SYMBOL

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THE RHYTHM OF YOUR HEARTBEATFollow in our instructor’s footsteps and, “Match your heartbeat to that of the land.” At the start of your day, repeat this quote three times. Then throughout your day think about every action and how you can modify it to give back to nature as nature has given to us.

SHARE YOUR SUSTAINABILITY STORYHow did you give back to nature? Share a photo of your sustainable actions on our Facebook page at facebook.com/ LakotaFundsCDFI.

 

 

End Notes 1 Kathleen A. Pickering and Benjamin Jewell, “Nature is Relative: Religious Affiliation, Environmental Attitudes, and Political Constraints on the Pine Ridge Indian Reservation”.

JSRNC 2:1. p.146, 2008. 2 Annie Heloise Abel, “Tabeau’s Narrative of Loisel’s Expedition to the Upper

Missouri”. Rose Abel

Wright, trans. Norman: University of Oklahoma Press. p.169, 1939.; Raymond J. DeMallie, ed., “Handbook of North American Indians”. vol. 13 part 2: Plains. William Sturtevant gen. ed. Washington D.C.: Smithsonian Institution. p.729, 731, 2001. 3 Kathleen A. Pickering and Benjamin Jewell, “Nature is Relative: Religious Affiliation, Environmental Attitudes, and Political Constraints on the Pine Ridge Indian Reservation”. JSRNC 2:1. p.142, 2008. 4 A. Leroi Gouran, “The flowers found with Shanidar IV, a Neanderthal burial in Iraq,” Science, vol.190, no. 4214, pp. 562-564, 1975.; Si-Yuan Pan, et al., “Historical Perspective of Traditional Indigenous Medical Practices: The current Renaissance and Conservation of Herbal Resources”. Evidence Based Complementary and Alternative Medicine. Vol 2014. Hindawi Publishing Corporation. p.1-2, 2014.

5 Si-Yuan Pan, et al., “Historical Perspective of Traditional Indigenous Medical Practices: The current Renaissance and Conservation of Herbal Resources”. Evidence Based Complementary and Alternative Medicine. Vol 2014. Hindawi Publishing Corporation. p.1, 2014. 6 UNICEF. Diarrhoea remains a leading killer of young children, despite the availability of a simple treatment solution. UNICEF Data: Monitoring the Situation of Children and Women, 2016. 7 Michael Grunwald. “The Swamp”. Simon and Schuster. ISBN 978-0-7432-5107-5. p. 25. 2007. 8

Richard Sherman, “Where the Heart Is”. 1 April, 2016. AARP. Accessed online: http://travel.aarp.

org/articles-tips/articles/info-2016/national-park-spiritual-connection.html. 14, July 2016.

Other Recommended Resources Gilmore, Melvin R. Uses of Plants by the Indians of the Missouri River Region, Enlarged Edition. Lincoln, NE: University of Nebraska Press, 2014.

Johnson, Timothy. CRC Ethnobotany Reference. Boca Raton, FL: CRC, 1999.

Kindscher, Kelly. Edible Wild Plants of the Prairie: An Ethnobotanical Guide. Lawrence, Kan.: U of Kansas, 1987.

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Kindscher, Kelly. Medicinal Wild Plants of the Prairie: An Ethnobotanical Guide. Lawrence, Kan.: U of Kansas, 1992. Moerman, Daniel E. Native American Ethnobotany. Portland: Timber Press, 1998.

Sherman, Richard T. Personal Contact. Email: rtsherman43@gmail.com Phone: 605-441-0272

 

 

Food for Thought Recipes Following are healthy, contemporary recipes that include traditional Lakota foods.

BUFFALO-DUMPLING SOUP Dumplings 1 lb. ground buffalo meat 2 eggs 2 slices of bread flour bread crumbs 1 grated carrot

1 chopped onion 3 tsp. chopped parsley salt pepper 2 Tbsp. vegetable oil

Place in bowl. Squeeze water out of bread and mix it with other ingredients in a bowl until you have a compact dough; if it is too soft, add breadcrumbs. Form dumplings (about 1-2 inches in diameter), place in boiling water, simmer on medium heat until dumplings swim to the top (only cooked dumplings do this). When finished, remove from water and place on a plate. Keep the dumpling broth and put it aside for the following soup recipe.

Soup Pour enough sunflower or olive oil into a soup pot to cover the bottom. Sauté 1 lb. cut carrots, 1 lb. cut wild turnips, 1 cup chopped lamb’s quarter, 1 sliced large leek, 1 cup chopped celery, 1 large chopped onion, 2 lbs. peeled and cut potatoes, 2 chopped wild onion pulp (or 1 normal onion), and Cheyenne turnip leaves. Add 1 tsp. turmeric, 1 pinch of fenugreek, ½ tsp. wild bergamot powder, ¼ tsp. calamus, salt and pepper; stir and add 2 quarts of water; simmer until vegetables are cooked, but not too soft (this enables the vegetables to still provide vitamins).

PRAIRIE POWER COCKTAIL 1 wild turnip 2 Jerusalem artichoke tubers ½ cup carrot juice

2 cups mineral water 1 cup apple juice honey

Peel Jerusalem artichokes and wild turnips, then wash and finely slice them. Cook these in mineral water (bottled water). Combine in a blender and strain through sieve. Add apple and carrot juice, and sweeten to taste with honey. If you add chili powder or Tabasco sauce, you will have a cocktail to delight your digestive system.

31

APPENDIX A

Ethnobotany of the Northern Plains Tribes

 

 

DELICIOUS 3 SISTERS 2 lb. chopped elk meat 1 lb. cubed squash 1 lb. corn 1 lb. cut green beans 3 lb. chopped potatoes

ground wild bergamot ground skunkbush leaf ground ginger cilantro leaf ground calamus ½ cup buffalo berries 1 Tbsp. chopped bohnenkraut 2 Tbsp. chopped stinging

nettle 2 Tbsp. cornmeal

CHEF’S DELIGHT 2 lbs. Jerusalem artichoke tubers 1 lb. potatoes ½ cup fresh lambs’ quarter

¼ cup fresh parsley ½ lb. ground buffalo meat 2 garlic cloves herbal salt or normal salt ¼ tsp. ground

Wash Jerusalem artichoke tubers and potatoes. Cook them unpeeled and whole until soft (make sure you do not overcook them!). Drain and peel potatoes, cut ends off Jerusalem artichokes, and then cut potatoes and Jerusalem artichokes in half. Slice each half into four pieces lengthwise. Heat oil or

or clarified butter

2 pints water 1 puffball or ½ lb. other mushrooms ½ cup chopped lamb’s quarter ½ lb. wild turnips 1 lb. potatoes ½ lb. Jerusalem artichokes 1 lb. cubed buffalo meat 1 Tbsp. chopped wild bergamot leaves 2 carrots ½ cup ground-plums

1 small squash 1 large onion rock salt and pepper 1 cup organic sunflower or olive oil 1 tsp. ground ginger ¼ tsp. ground fenugreek ½ tsp. ground coriander ¼ tsp. turmeric 1 Tbsp. flour pinch of cayenne pepper

Heat 1 cup (or more if needed) good quality vegetable oil and add all ingredients. Dust with cornmeal, stir, and add 4 cups (1 quart) water. Simmer at medium heat until meat is soft, adding more water if needed. Flavor to taste with salt and pepper.

PRAIRIE BUFFALO STEW

Peel and slice wild turnips, potatoes, and Jerusalem artichokes, and put all three into cold water to keep them from turning brown (add some drops of lemon juice). Dice carrots, slice ground-plums and mushrooms, and cut squash into small cubes. Heat oil and sauté chopped onions, add buffalo meat and flour and stir. After 5 minutes, add all vegetables and stir. Next, add wild bergamot, lamb’s quarter and all other herbs. Pour on water and let simmer until meat is tender. Salt to taste.

32

 

 

bl ack pepper 2 pinches ground caraway seeds ½ tsp. ground turmeric 1 cup organic cold pressed sunflower or olive oil

butter in pan and steam chopped garlic cloves, add potatoes and Jerusalem artichokes, and fry until golden brown. Stir in buffalo meat and season with herbal salt or regular salt and all spices. Add lambs’ quarter and parsley and fry for 5 more minutes.

STINGING NETTLE POWER SNACK Cover a slice of bread with butter and cover with fresh seeds of Stinging Nettle (early autumn). In the seed is the power of the whole plant. Attention: seeds of singing nettle do not burn.

BUFFALO BURGER DELUXE WITH ROASTED POTATOES AND CATTAIL-CUCUMBER DIP Burger Use same meat and vegetable mixture as for Buffalo Dumplings, plus 1 zucchini.

Soak bread in water. In the meantime sauté in 2 Tbsp. vegetable oil the chopped onion, carrot, zucchini and parsley, and place in bowl. Press water out of bread and add the vegetable mixture to the bowl. Knead until dough is fairly firm. If it is too soft, add some breadcrumbs. Shape dough into dumplings about 1-2 inches in diameter) and flatten into burgers. Cover both sides with flour and fry in hot oil.

Roasted Potatoes Boil potatoes in skins (Make sure you do not overcook them!). Peel and cut cooked potatoes into quarters lengthwise. Cover the bottom of a skillet with good quality vegetable oil, sauté chopped onion, add potatoes and sauté until the potatoes and onions are golden brown. Shake or turn over often. Add seasoning. Before serving, add fresh chopped lamb’s quarter.

Dip Mix 1 pkg. Philadelphia cream cheese with same amount of plain yogurt. Add 3 crushed garlic cloves, salt, pepper, small cubed cucumber and some cattail shoots.

Ethnobotany of the Northern Plains Tribes

Woyute Un Zanipi

If any questions or concerns arise in preparing one of the meals above, please contact Richard T. Sherman directly at: 605-441-0272 or rtsherman43@gmail.com.

33

These recipes by Christina Voormann and Charles White Buffalo with the great help of Richard T. Sherman as part of:

Battle Diabetes with Gifts from Mother Earth Lakota Food for Health

 

PH OTO COM MON NAM ESC IENT IFIC NAM EPH YSIC ALP ROPE RTIE SUS ES

Ga ther ing Mor eDa taCo mpl ete this cha rtw hile sea rchi ngfo rw ildfo odp lant s.

 

Plants List

 

 

 

Notes

38

 

 

 

Notes

Ethnobotany of the Northern Plains Tribes

39

 

 

Notes 40

 

 

 

Acknowledgments Lakota Funds is grateful for the opportunity to preserve and revitalize Lakota culture through the development of the Eth- nobotany of the Northern Plains Tribes course as part of the Building Native Industry Institute. Our staff worked diligently to see this project to fruition. We would also like to thank the following individuals and organizations who contributed to the project.

Wóphila Tȟáŋka (Big Thanks) Funding support was provided by the Administration for Na- tive Americans.

Richard T. Sherman brought his lifetime of experience and knowledge of plant life and the Lakota culture as our instruc- tor for the course. For any questions, comments, requests for tours, or other information regarding ethnobotany, hunting, and gathering, please contact Richard at 605-441-0272 or rtsherman43@gmail.com.

Fibonacci Productions delivered expert videography services and spent hours out on the prairie filming as well as in the lab editing in order to create a fabulous video.

With their in-depth knowledge of Lakota culture and the Pine Ridge Reservation, Sweet Grass Consulting developed curricu- lum content and activities for an enriching learning experience.

Cuny Communications applied exceptional creative ability to the design and development of the curriculum layout, as well as the online course. Their attention to detail allowed us to produce this superior final product.

 

 

PO Box 340 Kyle, SD 57752 (605) 455-2500 tel (605) 455-2585 fax

www.lakotafunds.org

 

  • Quick Start Guide
  • Opening Video
  • The Pine Ridge Reservation
    • MAP OF PINE RIDGE RESERVATION: HOME OF THE OGLALA LAKOTA
  • Quiz
  • Food Plants Video
  • Lakota Food
  • Gathering Data
  • Quiz
  • Medicinal Plants Video
  • Lakota Medicine
  • Native and Western: Making Connections
  • Quiz
    • WORD BANK
  • Ceremonial Plants Video
  • Lakota Ceremonies
  • Creating a Tobacco Mixture
    • MATCHING FOR YOUR MIXTURE
  • Quiz
  • Poisonous & Harmful Plants Video
  • Poison on the Prairie
    • DEADLY POISON: HEMLOCK
  • Creating a Memory Tool
  • Finding a Cure
  • Quiz
  • Sustainability Video
  • Sustaining Plant Life
  • Symbol of Sustainability
  • Other Recommended Resources
  • Food for Thought Recipes
    • Dumplings
    • Soup
    • Roasted Potatoes
    • Dip
  • Extra Fun: Gathering More Data
  • Plants List
  • Notes
  • Notes
  • Notes
 
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Biology

Endocrine Ed Online Interactive

Worksheet

 

HORMONES AND FEEDBACK

1. Most hormones are secreted by an ________________________________ gland.

2. How do hormones travel around the body? ____________________________________.

3. ________________ ______________ have receptors for the hormones.

4. ________________________ is a state of being in balance.

5. Once homeostasis is achieved, the ____________________ causes the production of the hormone to turn off. This is part of the negative feedback mechanism.

 

Complete the diagram as it relates to the process of growth in humans:

Pituitary gland ____________________________ ______________________ (effect)

 

 

 

Without feedback, what would happen to a person’s height? _____________________________

 

Questions:

1. How does a feedback loop work?

 

 

2. What would happen if negative feedback did not occur?

 

 

ADRENALINE AND STRESS

 

1. List several effects of adrenaline:

 

2. Adrenaline gets the body ready for ___________________________________________.

 

Game: control Ed’s adrenaline

What is one thing that will raise his adrenaline level?

 

Quiz:

1. In which situation would adrenaline most likely be secreted by the adrenal glands?

 

2. When sufficient adrenaline has been secreted, production stops. This is part of the mechanism known as _________________________ feedback.

 

MELATONIN AND SLEEP

 

1. Melatonin production is high when it is ______________________________.

2. Melatonin is secreted by the ___________________________ gland.

3. Do melatonin levels remain constant from the time you fall asleep until the time you wake up? _______________________

 

BLOOD SUGAR HORMONES

 

1. Most cells are affected by insulin; what three cell types are highlighted in this simulation that have insulin receptors?

 

 

 

2. Insulin causes cells to ____________________ sugar and therefore ________________ blood sugar levels.

3. Glucagon causes the ___________________ to release sugar and causes blood sugar levels to ____________________.

 

Game: When you exercise, blood sugar levels ________________________ causing the release of ____________________________ to bring those levels back to normal ranges.

 

FUN QUIZ

 

Matching: hormones to their function

 

_____ 1. Raises blood sugar A. adrenaline

_____ 2. Helps you sleep B. glucagon

_____ 3. Increases muscle strength C. insulin

_____ 4. Lowers blood sugar D. melatonin

5. Calcitonin lowers blood calcium adding that calcium to the _____________________.

6. The parathyroid _____________________ blood calcium by taking the calcium from the __________________.

7. Once homeostasis is reached, hormone release will be _________________________.

8. If blood sugar levels are too high, _____________________ is released.

9. If blood sugar levels are too low, ______________________ is released.

10. Antidiuretics (ADH) help the body retain water by affecting the ________________________ (organ).

11. You would most likely produce ADH when you are ____________________________.

 
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Genetics Take Home Test

Genetics 303 Dr. Joe Staton Fourth exam—take home Answer on separate paper, show all work, and be neat in the reporting of answers. STAPLE YOUR RESULTS! 1. In a human population, the genotype frequencies at one locus are 0.75 AA, 0.2 Aa, and 0.05 aa. What is the frequency

of the A allele [f(A)] and a allele [f(a)] for the population? Are they in Hardy-Weinberg equilibrium? 2. Calculate the number of heterozygotes in a population with p = 0.55 and q = 0.45 (at time = 0). After 4 generations of

inbreeding between siblings (F = 0.25) in a population of 1000. 3. Human albinism is an autosomal recessive trait. Suppose that you find an isolated village in the Andes where seven

people are albino. If the population size of the village was 783 and the population is in Hardy-Weinberg equilibrium with respect to this trait, how many individuals are expected to be carriers (heterozygotes)?

4. A boatload of Swedish tourists, all of whom bear the MM blood group, is marooned on Haldane Island, where they are

met by an equally sized population of Islanders, all bearing blood group NN. In time, the castaways become integrated into Island society. Assuming random mating, no mutation, no selection (based on blood group), and no genetic drift, what would you expect the blood group distribution to be among 1500 progeny of the new Haldane Island population?

5. You identify a population of mice (Peromyscus maniculatus) on an island. Their coat color is controlled by a single

gene: BB mice are black, Bb mice are gray, and bb mice are white. You take a census of the population and record the following numbers of mice:

Black 507 Gray 546 White 147 (a) What are the frequencies of the two alleles? (b) What are the Hardy-Weinberg equilibrium frequencies for these three phenotypes? (c) A heat wave hits the island. All 507 mice with black fur die from heat stroke, but the other mice survive. What are the

new allele frequencies for the population? (d) If the population suffers no further cataclysms after the heat wave, and the surviving animals mate randomly, what will

be the frequency of mice with black fur in the next generation? (e) If the climate is altered permanently, so that mice with black fur die before reproducing, which following statement is

correct? (1) At Hardy-Weinberg equilibrium, f(B) will equal 0.135. (2) The fitness of mice with gray fur (ωBb) must be equal to 0.5. (3) The fitness of mice with black fur (ωBB) is 0. (4) The B allele will disappear from the population in one generation. (5) The B allele will disappear from the population in two generations.

6. Which of the following are requirements for evolution by natural selection? Explain your answer. I Environmental change II Differential survival and reproduction III Heritability of phenotypic variation IV Variation in phenotype V Sexual reproduction

A) II, III, V B) II, III, IV C) I, II, IV D) III, IV, V E) II, IV, V

 

 

 

7. Which of the following processes is the source (origin) of genetic variation within populations? A) Reproductive Isolation B) Asexual reproduction C) Selection D) Mutation E) Genetic drift

Explain your answer including a description of what the others do to variation. 8. If the population (14,926 in 2013) of folks in Perry, GA, have an f(a) = 0.1 and folks in Valdosta, GA, has a f(a) = 0.7,

then how many people from Valdosta, GA, would have to migrate to Perry to increase the population to a f(a) = 0.15?

9. What is the Ne of a population with the following annual censuses, [note the drop in size due to 2005 being an extreme

drought year]? 2001: 10,000 2002: 9,700 2003: 8,800 2004: 4,600 2005: 700 2006: 2,400 2007: 3,800 2008: 7,650 2009: 8,400 2010: 9,700 2011: 10,110 2012: 11,060

10. Consider the following populations that have the genotypes shown in the following table: Population AA Aa aa 1 1.0 0.0 0.0 2 0.0 1.0 0.0 3 0.25 0.50 0.25 4 0.25 0.25 0.50 5 0.32 0.36 0.32 6 0.04 0.32 0.64 7 0.64 0.32 0.04 8 0.9025 0.095 0.0025

a. What are p and q for each population? b. Which of the populations are in Hardy-Weinberg equilibrium? c. Populations 1 and 2 have a tree fall across their islands so that individuals can cross. If equal numbers of the

individuals occur on each island, what is the new population’s allele frequencies and genotype frequencies after one generation of random mating?

d. In population 3, the a allele is less fit than the A allele, and the A allele is incompletely dominant. The result is that AA is perfectly fit (= 1.0), Aa has a fitness of 0.85, and aa has a fitness of 0.65. With no mutation or migration, graph the allele frequency of the a allele for 10 generations under selection (e.g., Time 0 = q above, Time 1 = first generation after selection)

e. In population 8, the population size gets radically reduced to 200 individuals, total. What is the most likely fate of the “a” allele, and what genetic principle would lead you to believe that the case?

 

 

11. You are given the following genetic data matrix of distances for crustaceans calculated for a region of the mtDNA called the 16S rDNA:

Brine Shrimp Striped-leg hermit King Crab Soldier crab Flat-claw hermit Long-clawed hermit Brine Shrimp ─ Striped-leg hermit 0.354 ─ King Crab 0.309 0.260 ─ Soldier crab (hermit) 0.321 0.268 0.067 ─ Flat-claw hermit 0.337 0.245 0.108 0.111 ─ Long-clawed hermit 0.312 0.249 0.090 0.096 0.044 ─ Calculate the average distance and draw the resulting UPGMA tree based on these distances. Write a brief interpretation of the branching pattern in the tree. 12. You digest a linear piece of DNA with two restriction enzymes, BamH1 & Sma1, and get the following sized fragments (in kb [kilobases]): BamHI SmaI BamHI & SmaI 13 kb 11 kb 10 kb 6 kb 5 kb 5 kb 3 kb 3 kb 1 kb Draw the appropriate restriction fragment map based on this data labeling all restriction sites. Extra Credit: You have a population of gribbets in captivity where the adults have a genetic frequency of f(A)=0.5 and f(a) = 0.5. When randomly mated, they have offspring that are represented in the following frequencies: f(AA) f(Aa) f(aa) 0.194444 0.555556 0.25 But you notice that the total generational reproduction level is only 90% that of wild populations (i.e. the ω is only 0.9). Work backwards to figure out the type of condition from table 25.5, and calculate the equilibrium frequency for the a allele (q). Give it a try! Partial credit for attempts…

 

 
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Lab Enzymes

Full Name:

 

UMUC Biology 102/103

Lab 4: Enzymes

INSTRUCTIONS:

 

        On your own and without assistance, complete this Lab 4Answer Sheet electronically and submit it via the Assignments Folder by the date listed intheCourse Schedule (underSyllabus).

        To conduct your laboratory exercises, use the Laboratory Manual located under Course Content. Read the introduction and the directions for each exercise/experiment carefully before completing the exercises/experiments and answering the questions.

        Save your Lab 4Answer Sheet in the following format:  LastName_Lab4 (e.g., Smith_Lab4).

        You should submit your document as a Word (.doc or .docx) or Rich Text Format (.rtf) file for best compatibility.

 

Pre-Lab Questions

 

1.      How could you test to see if an enzyme was completely saturated during an experiment?

 

2.      List three conditions that would alter the activity of an enzyme. Be specific with your explanation.

 

3.      Take a look around your house and identify household products that work by means of an enzyme. Name the products, and indicate how you know they work with an enzyme.

 

 

Experiment 1: Enzymes in Food

This experiment tests for the presence of amylase in food by using Iodine-Potassium Iodide, IKI. IKI is a color indicator used to detect starch. This indicator turns dark purple or black in color when in the presence of starch. Therefore, if the IKI solution turns to a dark purple or black color during the experiment, one can determine that amylase is not present (because presence of amylase would break down the starch molecules, and the IKI would not change color).

 

Materials

(1) 2 oz. Bottle (Empty)

(1) 100 mL Graduated Cylinder

30 mL Iodine-Potassium Iodide, IKI

Permanent Marker

Ruler

2 Spray Lids

30 mL Starch (liquid)

*Cutting Board

 

*2 Food Products (e.g., ginger root, apple, potato, etc.)

*Kitchen Knife

*Paper Towel

*Saliva Sample

*Tap Water

*You Must Provide

 

Procedure:

1.      Remove the cap from the starch solution. Attach the spray lid to the starch solution.

2.      Rinse out the empty two ounce bottle with tap water. Use the 100 mL graduated cylinder to measure and pour 30 mL of IKI into the empty two ounce bottle. Attach the remaining spray lid to the bottle.

3.      Set up a positive control for this experiment by spraying a paper towel with the starch solution. Allow the starch to dry for approximately one hour (this time interval may vary by location).

4.      In the mean time, set up a negative control for this experiment. Use your knowledge of the scientific method and experimental controls to establish this component (hint: what should happen when IKI solution contacts something that does not contain starch?) Identify your negative control in Table 1.

Note: Be sure to space the positive and negative controls apart from each other to prevent cross-contamination.

5.      When the starch solution has dried, test your positive and negative controls. This step establishes a baseline color scale for you to evaluate the starch concentration of the food products you will test in Steps 7 – 11. Record your results in Table 1.

6.      Select two food items from your kitchen cabinet or refrigerator.

7.      Obtain a kitchen knife and a cutting board. Carefully cut your selected food items to create a fresh surface.

Figure 3: Sample set-up.

8.      Gently rub the fresh/exposed area of the food items on the dry, starch-sprayed paper towel back and forth 10 – 15 times. Label where each specimen was rubbed on the paper towel with a permanent marker (Figure 3).

9.      Wash your hands with soap and water.

10.  Take your finger and place it on your tongue to transfer some saliva to your finger. Then, rub your moistened finger saliva into the paper towel. Repeat this step until you are able to adequately moisten the paper towel.

Note: You should always wash your hands before touching your tongue! Alternatively, if you do not wish to put your hands in your mouth, you may also provide a saliva sample by spitting in a separate bowl and rubbing the paper towel in the saliva. Be sure not to spit on the paper towel directly as you may unintentionally cross-contaminate your samples.

11.  Wait five minutes.

12.  Hold the IKI spray bottle 25 – 30 cm away from the paper towel, and mist with the IKI solution.

13.  The reaction will be complete after approximately 60 seconds. Observe where color develops, and consider what these results indicate. Record your results in Table 1.

Table 1: Substance vs. Starch Presence
Substance Resulting Color Presence of Starch?
Positive Control: Starch    
Negative Control: Student Must Select    
Food Product:    
Food Product:    
Saliva:    

 

Post-Lab Questions

1.      What were your controls for this experiment? What did they demonstrate? Why was saliva included in this experiment?

 

2.      What is the function of amylase? What does amylase do to starch?

 

3.      Which of the foods that you tested contained amylase? Which did not? What experimental evidence supports your claim?

 

4.      Saliva does not contain amylase until babies are two months old. How could this affect an infant’s digestive requirements?

 

5.     There is another digestive enzyme (other than salivary amylase) that is secreted by the salivary glands. Research to determine what this enzyme is called. What substrate does it act on? Where in the body does it become activated, and why?

 

6.       Digestive enzymes in the gut include proteases, which digest proteins. Why don’t these enzymes digest the stomach and small intestine, which are partially composed of protein?

 

 

Experiment 2: Effect of Temperature on Enzyme Activity

Yeast cells contain catalase, an enzyme which helps convert hydrogen peroxide to water

Figure 4: Catalase catalyzes the decomposition of hydrogen peroxide to water and oxygen.

and oxygen. This enzyme is very significant as hydrogen peroxide can be toxic to cells if allowed to accumulate. The effect of catalase can be seen when yeast is combined with hydrogen peroxide (Catalase: 2 H2O2 → 2 H2O + O2).

In this lab you will examine the effects of temperature on enzyme (catalase) activity based on the amount of oxygen produced. Note, be sure to remain observant for effervescence when analyzing your results.

 

Materials

(2) 250 mL Beakers

3 Balloons

30 mL 3% Hydrogen Peroxide, H2O2

Measuring Spoon

Permanent Marker

Ruler

20 cm String

 

3 Test Tubes (Glass)

Test Tube Rack

Thermometer

Yeast Packet

*Hot Water Bath

*Stopwatch

*You Must Provide

 

Procedure

1.      Use a permanent marker to label test tubes 1, 2, and 3. Place them in the test tube rack.

2.      Fill each tube with 10 mL hydrogen peroxide. Then, keep one of the test tubes in the test tube rack, but transfer the two additional test tubes to two separate 250 mL beakers.

3.      Find one of the balloons, and the piece of string. Wrap the string around the uninflated balloon and measure the length of the string with the ruler. Record the measurement in Table 2.

4.      Create a hot water bath by performing the following steps:

1.      Determine if you will use a stovetop or microwave to heat the water. Use the 100 mL graduated cylinder to measure and pour approximately 200 mL of water into a small pot or microwave-safe bowl (you will have to measure this volume in two separate allocations).

2.      If using a stovetop, obtain a small pot and proceed to Step 4c. If using a microwave, obtain a microwave-safe bowl and proceed to Step 4e.

3.      If using a stove, place a small pot on the stove and turn the stove on to a medium heat setting.

4.      Carefully monitor the water in the pot until it comes to a soft boil (approximately 100 °C). Use the thermometer provided in your lab kit to verify the water temperature. Turn the stove off when the water begins to boil. Immediately proceed to Step 5.

CAUTION: Be sure to turn the stove off after creating the hot water bath. Monitor the heating water at all times, and never handle a hot pan without appropriate pot holders.

5.      If using a microwave, place the microwave-safe bowl in the microwave and heat the water in 30 second increments until the temperature of the water is approximately 100 °C. Use the thermometer provided in your lab kit to verify the water temperature. Wait approximately one minute before proceeding to Step 5.

5.      Place Tube 1 in the refrigerator. Leave Tube 2 at room temperature, and place Tube 3 in the hot water bath.

Important Note: The water should be at approximately 85 °C when you place Tube 3 in it. Verify the temperature with the thermometer to ensure the water is not too hot! Temperatures which exceed approximately 85  °C may denature the hydrogen peroxide.

6.      Record the temperatures of each condition in Table 2. Be sure to provide the thermometer with sufficient time in between each environment to avoid obscuring the temperature readings.

7.      Let the tubes sit for 15 minutes.

8.      During the 15 minutes prepare the balloons with yeast by adding ¼ tsp. of yeast each balloon. Make sure all the yeast gets settled to the bulb of the balloon and not caught in the neck. Be sure not spill yeast while handling the balloons.

9.      Carefully stretch the neck of the balloon to help ensure it does not rip when stretched over the opening of the test tube.

10.  Attach the neck of a balloon you prepared in step 8 to the top of Tube 2 (the room temperature test tube) making sure to not let the yeast spill into the test tube yet. Once the balloon is securely attached to the test tube lift the balloon and allow the yeast to enter the test tube. Tap the bulb of the balloon to ensure all the yeast falls into the tube.

11.  As quickly and carefully as possible remove the Tube 1 (cold) from the refrigerator and repeat steps 9 – 10 with Tube 1 using a balloon you prepared in step 8.

12.  As quickly and carefully as possible remove Tube 3 (hot) from the hot water bath and repeat steps 9 – 10 with Tube 3 using a balloon you prepared in step 8.

13.  Swirl each tube to mix, and wait 30 seconds.

14.  Wrap the string around the center of each balloon to measure the circumference. Measure the length of string with a ruler. Record your measurements in Table 2.

Table 2: Balloon Circumference vs. Temperature
Tube Temperature (°C) Balloon Circumference (Uninflated; cm) Balloon Circumference (Final; cm)
1 – (Cold)      
2 – (RT)    
3 – (Hot)    

 

 

Post-Lab Questions

1.      What reaction is being catalyzed in this experiment?

2.      What is the enzyme in this experiment? What is the substrate?

3.      What is the independent variable in this experiment? What is the dependent variable?

4.      How does the temperature affect enzyme function? Use evidence from your data to support your answer.

 

5.      Draw a graph of balloon diameter vs. temperature. What is the correlation?

 

6.      Is there a negative control in this experiment? If yes, identify the control. If no, suggest how you could revise the experiment to include a negative control.

 

7.      In general, how would an increase in substrate alter enzyme activity? Draw a graph to illustrate this relationship.

 

8.      Design an experiment to determine the optimal temperature for enzyme function, complete with controls. Where would you find the enzymes for this experiment? What substrate would you use?

 
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