LAB 3 Quantification Cultures Mircoorganism
Lab 3 Quantification of Cultured Microorganisms “BIO250L”
Student Name: Click here to enter text.
Kit Code (located on the lid of your lab kit): Click here to enter text.
“Experiment 1- Direct Counts Following Serial Dilution”
“Table 1: Experiment 1 Growth Results”
| “Plate” | “Classification” | “CFU/plate” |
| “10-1” | Click here to enter text. | Click here to enter text. |
| “10-2” | Click here to enter text. | Click here to enter text. |
| “10-3” | Click here to enter text. | Click here to enter text. |
| “10-4” | Click here to enter text. | Click here to enter text. |
| “10-5” | Click here to enter text. | Click here to enter text. |
| “10-6” | Click here to enter text. | Click here to enter text. |
“Post-Lab Questions”
“1. What was the population density of the original sample? What would have happened if you had inoculated an agar plate with 1mL of the original sample?”
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“2. Did all the bacterial colonies on the countable plate(s) have a similar appearance? If not, how would you explain this?”
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“3. You have performed a serial dilution of an unknown sample and counted 73 CFU on a countable plate that was marked as a 10-4 dilution and you used 0.1mL to inoculate the plate. What is the population density of the original sample?”
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“4. List at least one advantage and one disadvantage to the direct plate counting method following serial dilution for determining bacterial concentration.”
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“5. Compare and contrast direct plate counts for bacterial and viral populations.”
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“6. What are some sources of error in the serial dilution/direct plate counting method?”
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“Insert photo of your cultures after incubation with your name clearly visible in the background:”
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