Microscopy For Microbiology – Use And Function Hands-On Labs, Inc. Version 42-0249-00-02

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Microscopy for Microbiology – Use and Function

Hands-On Labs, Inc. Version 42-0249-00-02

Lab Report Assistant

This document is not meant to be a substitute for a formal laboratory report. The Lab Report Assistant is simply a summary of the experiment’s questions, diagrams if needed, and data tables that should be addressed in a formal lab report. The intent is to facilitate students’ writing of lab reports by providing this information in an editable file which can be sent to an instructor.

Exercise 1: Getting to Know your Compound Microscope

Data Table 1. Microscope Components.

Letter Component Name Component Function
A    
B    
C    
D    
E    
F    
G    
H    
I    
J    
K    
L    
M    

 

Data Table 2. Total Magnification.

Lens Ocular Magnification Objective Magnification Total Magnification
Scanning      
Low Power      
High Power      
Oil Immersion      

Data Table 3. Field of View.

Lens Total Magnification Field of View (mm) Field of View (µM)
Scanning      
Low Power      
High Power      
Oil Immersion      

 

Data Table 4. Letter e Viewing Results.

Lens Photograph Observations
Scanning    
Low    
High    
Oil Immersion    

 

Questions

A. Describe the details in the slides “Letter e” that become visible as the power changed from scanning power, to low power, to high power.

B. Why is it important to calculate the diameter of the field when first using the microscope?

Exercise 2: Viewing Prepared Microbe Slides

Data Table 5. Prepared Slide Viewing Results.

Slide Photograph Total Magnification
Amoeba  

 

 

  
Penicillium  

 

  
Yeast  

 

 

  
Spirillium  

 

 

  
Bacillus  

 

 

  
Coccus  

 

 

  

 

Questions

A. Using the field of view calculated in Exercise 1 for the high power lens, approximately how far across are each of the cells in the Bacteria Coccus Form slide in Data Table 5? Show your calculations.

B. Detail techniques you found helpful for focusing on the various slides in this exercise.

Exercise 3: Preparing Wet-Mount Slides

Data Table 6. Wet-Mount Viewing Results.

Slide Photograph Total Magnification
Cheek Cell Smear  

 

 

  
Dental Tatar Smear  

 

 

  

 

Questions

A. Describe the similarities and differences between the cheek cell wet mount and dental plaque wet mount.

B. How did the process of preparing wet-mount slides become easier as you prepared the second wet-mount slide of this exercise?

 
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